anti arginase1 apc Search Results


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Bio-Techne corporation human/mouse arginase 1/arg1 fluorescein-conjugated antibody
Human/Mouse Arginase 1/Arg1 Fluorescein Conjugated Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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R&D Systems allophycocyanin apc conjugated anti arginase 1
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R&D Systems apc conjugated anti arg 1
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R&D Systems anti human arg 1 apc
Anti Human Arg 1 Apc, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Novus Biologicals polyclonal apc anti arginase 1 antibody
Polyclonal Apc Anti Arginase 1 Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Santa Cruz Biotechnology apc labeled arginase1
Figure 3 Flow cytometry and qRT/PCR analyses of immune cell infiltrates. (A) Flow cytometry gating strategy for macrophages (CD11b +CD45hiLy6G-), microglia (CD11b+CD45loLy6G-), and neutrophils (CD11b+CD45hiLy6G+). (B) Macrophages or microglia were further characterized as alternatively activated (M2) <t>CD206+Ym1+Arg1+</t> or (C) classically activated (M1) CD86+MHCII+iNOS+. (D) Flow cytometry gating for T cell subsets: Th1 = CD4+IFN-γ+, Th17 = CD4+IL17+, Treg = CD4+CD25+FOXP3. (E, F, G) Flow cytometry of cells isolated from pooled brain and spinal cord showed that the only significant difference in total numbers of T cell subtypes isolated from the CNS at 21 dpi was a decrease in the ratio of IFN-γ+ to IL-17+ cells in the astroglial CXCL10 knockout mice (n = 3 mice/group, P = 0.0063). (H, I) No significant differences between astroglial CXCL10 knockout and control mice in total numbers of macrophages, microglia, and neutrophils (H) or M1 and M2 subtypes of macrophages and microglia (I) isolated from the CNS at 21 dpi (n = 3). (J) qRT/PCR of spinal cord tissue isolated at 14 dpi normalized to the housekeeping gene HSP90; CXCR3 expression was significantly upregulated in astroglial CXCL10 knockout mice compared to controls (P = 0.0157). IFN-γ, FOXP3, RORγt, iNOS, and arginase-1 mRNA levels were not significantly different between astroglial CXCL10 knockout and control mice at 14 dpi (n = 6 mice/group). Vertical bars = SEMs.
Apc Labeled Arginase1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bio-Techne corporation human/mouse arginase 1/arg1 pe-conjugated antibody
Figure 3 Flow cytometry and qRT/PCR analyses of immune cell infiltrates. (A) Flow cytometry gating strategy for macrophages (CD11b +CD45hiLy6G-), microglia (CD11b+CD45loLy6G-), and neutrophils (CD11b+CD45hiLy6G+). (B) Macrophages or microglia were further characterized as alternatively activated (M2) <t>CD206+Ym1+Arg1+</t> or (C) classically activated (M1) CD86+MHCII+iNOS+. (D) Flow cytometry gating for T cell subsets: Th1 = CD4+IFN-γ+, Th17 = CD4+IL17+, Treg = CD4+CD25+FOXP3. (E, F, G) Flow cytometry of cells isolated from pooled brain and spinal cord showed that the only significant difference in total numbers of T cell subtypes isolated from the CNS at 21 dpi was a decrease in the ratio of IFN-γ+ to IL-17+ cells in the astroglial CXCL10 knockout mice (n = 3 mice/group, P = 0.0063). (H, I) No significant differences between astroglial CXCL10 knockout and control mice in total numbers of macrophages, microglia, and neutrophils (H) or M1 and M2 subtypes of macrophages and microglia (I) isolated from the CNS at 21 dpi (n = 3). (J) qRT/PCR of spinal cord tissue isolated at 14 dpi normalized to the housekeeping gene HSP90; CXCR3 expression was significantly upregulated in astroglial CXCL10 knockout mice compared to controls (P = 0.0157). IFN-γ, FOXP3, RORγt, iNOS, and arginase-1 mRNA levels were not significantly different between astroglial CXCL10 knockout and control mice at 14 dpi (n = 6 mice/group). Vertical bars = SEMs.
Human/Mouse Arginase 1/Arg1 Pe Conjugated Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
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R&D Systems sheep anti arginase 1
Figure 3 Flow cytometry and qRT/PCR analyses of immune cell infiltrates. (A) Flow cytometry gating strategy for macrophages (CD11b +CD45hiLy6G-), microglia (CD11b+CD45loLy6G-), and neutrophils (CD11b+CD45hiLy6G+). (B) Macrophages or microglia were further characterized as alternatively activated (M2) <t>CD206+Ym1+Arg1+</t> or (C) classically activated (M1) CD86+MHCII+iNOS+. (D) Flow cytometry gating for T cell subsets: Th1 = CD4+IFN-γ+, Th17 = CD4+IL17+, Treg = CD4+CD25+FOXP3. (E, F, G) Flow cytometry of cells isolated from pooled brain and spinal cord showed that the only significant difference in total numbers of T cell subtypes isolated from the CNS at 21 dpi was a decrease in the ratio of IFN-γ+ to IL-17+ cells in the astroglial CXCL10 knockout mice (n = 3 mice/group, P = 0.0063). (H, I) No significant differences between astroglial CXCL10 knockout and control mice in total numbers of macrophages, microglia, and neutrophils (H) or M1 and M2 subtypes of macrophages and microglia (I) isolated from the CNS at 21 dpi (n = 3). (J) qRT/PCR of spinal cord tissue isolated at 14 dpi normalized to the housekeeping gene HSP90; CXCR3 expression was significantly upregulated in astroglial CXCL10 knockout mice compared to controls (P = 0.0157). IFN-γ, FOXP3, RORγt, iNOS, and arginase-1 mRNA levels were not significantly different between astroglial CXCL10 knockout and control mice at 14 dpi (n = 6 mice/group). Vertical bars = SEMs.
Sheep Anti Arginase 1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bio-Techne corporation mouse ym1/chitinase 3-like 3 biotinylated antibody
Figure 3 Flow cytometry and qRT/PCR analyses of immune cell infiltrates. (A) Flow cytometry gating strategy for macrophages (CD11b +CD45hiLy6G-), microglia (CD11b+CD45loLy6G-), and neutrophils (CD11b+CD45hiLy6G+). (B) Macrophages or microglia were further characterized as alternatively activated (M2) <t>CD206+Ym1+Arg1+</t> or (C) classically activated (M1) CD86+MHCII+iNOS+. (D) Flow cytometry gating for T cell subsets: Th1 = CD4+IFN-γ+, Th17 = CD4+IL17+, Treg = CD4+CD25+FOXP3. (E, F, G) Flow cytometry of cells isolated from pooled brain and spinal cord showed that the only significant difference in total numbers of T cell subtypes isolated from the CNS at 21 dpi was a decrease in the ratio of IFN-γ+ to IL-17+ cells in the astroglial CXCL10 knockout mice (n = 3 mice/group, P = 0.0063). (H, I) No significant differences between astroglial CXCL10 knockout and control mice in total numbers of macrophages, microglia, and neutrophils (H) or M1 and M2 subtypes of macrophages and microglia (I) isolated from the CNS at 21 dpi (n = 3). (J) qRT/PCR of spinal cord tissue isolated at 14 dpi normalized to the housekeeping gene HSP90; CXCR3 expression was significantly upregulated in astroglial CXCL10 knockout mice compared to controls (P = 0.0157). IFN-γ, FOXP3, RORγt, iNOS, and arginase-1 mRNA levels were not significantly different between astroglial CXCL10 knockout and control mice at 14 dpi (n = 6 mice/group). Vertical bars = SEMs.
Mouse Ym1/Chitinase 3 Like 3 Biotinylated Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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Bio-Techne corporation nbp2-67716
Figure 3 Flow cytometry and qRT/PCR analyses of immune cell infiltrates. (A) Flow cytometry gating strategy for macrophages (CD11b +CD45hiLy6G-), microglia (CD11b+CD45loLy6G-), and neutrophils (CD11b+CD45hiLy6G+). (B) Macrophages or microglia were further characterized as alternatively activated (M2) <t>CD206+Ym1+Arg1+</t> or (C) classically activated (M1) CD86+MHCII+iNOS+. (D) Flow cytometry gating for T cell subsets: Th1 = CD4+IFN-γ+, Th17 = CD4+IL17+, Treg = CD4+CD25+FOXP3. (E, F, G) Flow cytometry of cells isolated from pooled brain and spinal cord showed that the only significant difference in total numbers of T cell subtypes isolated from the CNS at 21 dpi was a decrease in the ratio of IFN-γ+ to IL-17+ cells in the astroglial CXCL10 knockout mice (n = 3 mice/group, P = 0.0063). (H, I) No significant differences between astroglial CXCL10 knockout and control mice in total numbers of macrophages, microglia, and neutrophils (H) or M1 and M2 subtypes of macrophages and microglia (I) isolated from the CNS at 21 dpi (n = 3). (J) qRT/PCR of spinal cord tissue isolated at 14 dpi normalized to the housekeeping gene HSP90; CXCR3 expression was significantly upregulated in astroglial CXCL10 knockout mice compared to controls (P = 0.0157). IFN-γ, FOXP3, RORγt, iNOS, and arginase-1 mRNA levels were not significantly different between astroglial CXCL10 knockout and control mice at 14 dpi (n = 6 mice/group). Vertical bars = SEMs.
Nbp2 67716, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bio-Techne corporation sirt4 antibody
Figure 3 Flow cytometry and qRT/PCR analyses of immune cell infiltrates. (A) Flow cytometry gating strategy for macrophages (CD11b +CD45hiLy6G-), microglia (CD11b+CD45loLy6G-), and neutrophils (CD11b+CD45hiLy6G+). (B) Macrophages or microglia were further characterized as alternatively activated (M2) <t>CD206+Ym1+Arg1+</t> or (C) classically activated (M1) CD86+MHCII+iNOS+. (D) Flow cytometry gating for T cell subsets: Th1 = CD4+IFN-γ+, Th17 = CD4+IL17+, Treg = CD4+CD25+FOXP3. (E, F, G) Flow cytometry of cells isolated from pooled brain and spinal cord showed that the only significant difference in total numbers of T cell subtypes isolated from the CNS at 21 dpi was a decrease in the ratio of IFN-γ+ to IL-17+ cells in the astroglial CXCL10 knockout mice (n = 3 mice/group, P = 0.0063). (H, I) No significant differences between astroglial CXCL10 knockout and control mice in total numbers of macrophages, microglia, and neutrophils (H) or M1 and M2 subtypes of macrophages and microglia (I) isolated from the CNS at 21 dpi (n = 3). (J) qRT/PCR of spinal cord tissue isolated at 14 dpi normalized to the housekeeping gene HSP90; CXCR3 expression was significantly upregulated in astroglial CXCL10 knockout mice compared to controls (P = 0.0157). IFN-γ, FOXP3, RORγt, iNOS, and arginase-1 mRNA levels were not significantly different between astroglial CXCL10 knockout and control mice at 14 dpi (n = 6 mice/group). Vertical bars = SEMs.
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NSJ Bioreagents cd4 antibody
Figure 3 Flow cytometry and qRT/PCR analyses of immune cell infiltrates. (A) Flow cytometry gating strategy for macrophages (CD11b +CD45hiLy6G-), microglia (CD11b+CD45loLy6G-), and neutrophils (CD11b+CD45hiLy6G+). (B) Macrophages or microglia were further characterized as alternatively activated (M2) <t>CD206+Ym1+Arg1+</t> or (C) classically activated (M1) CD86+MHCII+iNOS+. (D) Flow cytometry gating for T cell subsets: Th1 = CD4+IFN-γ+, Th17 = CD4+IL17+, Treg = CD4+CD25+FOXP3. (E, F, G) Flow cytometry of cells isolated from pooled brain and spinal cord showed that the only significant difference in total numbers of T cell subtypes isolated from the CNS at 21 dpi was a decrease in the ratio of IFN-γ+ to IL-17+ cells in the astroglial CXCL10 knockout mice (n = 3 mice/group, P = 0.0063). (H, I) No significant differences between astroglial CXCL10 knockout and control mice in total numbers of macrophages, microglia, and neutrophils (H) or M1 and M2 subtypes of macrophages and microglia (I) isolated from the CNS at 21 dpi (n = 3). (J) qRT/PCR of spinal cord tissue isolated at 14 dpi normalized to the housekeeping gene HSP90; CXCR3 expression was significantly upregulated in astroglial CXCL10 knockout mice compared to controls (P = 0.0157). IFN-γ, FOXP3, RORγt, iNOS, and arginase-1 mRNA levels were not significantly different between astroglial CXCL10 knockout and control mice at 14 dpi (n = 6 mice/group). Vertical bars = SEMs.
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Image Search Results


Figure 3 Flow cytometry and qRT/PCR analyses of immune cell infiltrates. (A) Flow cytometry gating strategy for macrophages (CD11b +CD45hiLy6G-), microglia (CD11b+CD45loLy6G-), and neutrophils (CD11b+CD45hiLy6G+). (B) Macrophages or microglia were further characterized as alternatively activated (M2) CD206+Ym1+Arg1+ or (C) classically activated (M1) CD86+MHCII+iNOS+. (D) Flow cytometry gating for T cell subsets: Th1 = CD4+IFN-γ+, Th17 = CD4+IL17+, Treg = CD4+CD25+FOXP3. (E, F, G) Flow cytometry of cells isolated from pooled brain and spinal cord showed that the only significant difference in total numbers of T cell subtypes isolated from the CNS at 21 dpi was a decrease in the ratio of IFN-γ+ to IL-17+ cells in the astroglial CXCL10 knockout mice (n = 3 mice/group, P = 0.0063). (H, I) No significant differences between astroglial CXCL10 knockout and control mice in total numbers of macrophages, microglia, and neutrophils (H) or M1 and M2 subtypes of macrophages and microglia (I) isolated from the CNS at 21 dpi (n = 3). (J) qRT/PCR of spinal cord tissue isolated at 14 dpi normalized to the housekeeping gene HSP90; CXCR3 expression was significantly upregulated in astroglial CXCL10 knockout mice compared to controls (P = 0.0157). IFN-γ, FOXP3, RORγt, iNOS, and arginase-1 mRNA levels were not significantly different between astroglial CXCL10 knockout and control mice at 14 dpi (n = 6 mice/group). Vertical bars = SEMs.

Journal: Journal of neuroinflammation

Article Title: Deletion of astroglial CXCL10 delays clinical onset but does not affect progressive axon loss in a murine autoimmune multiple sclerosis model.

doi: 10.1186/1742-2094-11-105

Figure Lengend Snippet: Figure 3 Flow cytometry and qRT/PCR analyses of immune cell infiltrates. (A) Flow cytometry gating strategy for macrophages (CD11b +CD45hiLy6G-), microglia (CD11b+CD45loLy6G-), and neutrophils (CD11b+CD45hiLy6G+). (B) Macrophages or microglia were further characterized as alternatively activated (M2) CD206+Ym1+Arg1+ or (C) classically activated (M1) CD86+MHCII+iNOS+. (D) Flow cytometry gating for T cell subsets: Th1 = CD4+IFN-γ+, Th17 = CD4+IL17+, Treg = CD4+CD25+FOXP3. (E, F, G) Flow cytometry of cells isolated from pooled brain and spinal cord showed that the only significant difference in total numbers of T cell subtypes isolated from the CNS at 21 dpi was a decrease in the ratio of IFN-γ+ to IL-17+ cells in the astroglial CXCL10 knockout mice (n = 3 mice/group, P = 0.0063). (H, I) No significant differences between astroglial CXCL10 knockout and control mice in total numbers of macrophages, microglia, and neutrophils (H) or M1 and M2 subtypes of macrophages and microglia (I) isolated from the CNS at 21 dpi (n = 3). (J) qRT/PCR of spinal cord tissue isolated at 14 dpi normalized to the housekeeping gene HSP90; CXCR3 expression was significantly upregulated in astroglial CXCL10 knockout mice compared to controls (P = 0.0157). IFN-γ, FOXP3, RORγt, iNOS, and arginase-1 mRNA levels were not significantly different between astroglial CXCL10 knockout and control mice at 14 dpi (n = 6 mice/group). Vertical bars = SEMs.

Article Snippet: For macrophage subtypes and neutrophils, cells were stained brilliant violet (BV)711-labeled anti-mouse CD11b, PB-labeled Ly6G, PE-labeled Ly6C, PE-Cy7-labeled anti-mouse CD11c, PE-labeled IL-6, PE-Cy7 labeled TNFα, (BD Bioscience), APC-labeled F4/ 80, PE-labeled CD206, BV510-labeled CD86, PE-Cy7labeled CD45, APC-Cy7-labeled MHCII (Biolegend), APC-labeled arginase1, biotin streptavidin-labeled Ym1 (R&D Systems), Peridinin-chlorophyll-protein complexcyanine 5.5 (PerCP-Cy5.5)-labeled iNOS (Santa Cruz Biotechnology).

Techniques: Flow Cytometry, Quantitative RT-PCR, Isolation, Knock-Out, Control, Expressing